Which stain is used to stain nucleus and chromosomes?
Methylene green used to stain Golgi bodies, pectic substances and also nuclei. Carmine is the basic dye used to stain nucleic acid and chromosomes and gives them pink colour. Safranin is used as counterstain in gram staining and endospore staining.
Which stain is used to stain nucleus?
Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.
Which stains are used to stain chromosomes?
Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining.
What are the stains used to stain chromosomes and chromatin?
Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.
What is Acetocarmine stain?
Acetocarmine is a non-specific nuclear stain which simply binds the chromosomes and gives colour to them. However, specific nuclear stain (e.g. feulgen) reacts with chromosomes to give them the colour. Acetocarmine is a dye obtained from insects.
How is a nucleus stained?
The nucleus contains a large amount of nucleic acid, so a reagent that binds to nucleic acids is used. Some dyes for nuclear staining can stain the nucleus of living cells while others require cells to be fixed and stained. When performing immunofluorescence staining of fixed cells, DAPI can be used as a nuclear stain.
What is DAPI staining used for?
DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy.
Which of the following stain is used for staining?
Basic stains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged.
Which stain is usually used to colour chromosomes Class 11?
Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.