Which dye is best for staining chromosome?
The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine. Carmine is a basic dye which is obtained from the offspring insect.
Which of the following stain is used for staining chromosomes?
Carmine is the basic dye used to stain nucleic acid and chromosomes and gives them pink colour. Safranin is used as counterstain in gram staining and endospore staining. Safranin can also be used for detection of cartilage and mast cell granules.
Which is best suited staining for karyotype analysis chromosomes?
The first method to be used to identify all 46 human chromosomes was Q-banding (Figure 1b), which is achieved by staining the chromosomes with quinacrine and examining them under UV light. This method is most useful for examining chromosomal translocations, especially ones involving the Y chromosome.
Which of the following stain is used to stain nucleus and chromosomes?
Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.
Which is the best suited stain in order to study chromosome?
Answer: Caramine is best suited for staining chromosomes.
Which dye is not used to stain chromosomes?
(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.
What are the stains used to stain chromosomes and chromatin?
Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.
What is Acetocarmine stain?
Acetocarmine is a non-specific nuclear stain which simply binds the chromosomes and gives colour to them. However, specific nuclear stain (e.g. feulgen) reacts with chromosomes to give them the colour. Acetocarmine is a dye obtained from insects.
Which of the following stain is used to study the karyotype analysis?
The contemporary method of Giemsa staining (G-banding) allows the resolution of at least 400 to 800 different bands on all chromosomes. … Therefore, karyotyping is used to detect major structural abnormalities of the chromosomes.
How do you identify chromosomes in a karyotype?
In a given species, chromosomes can be identified by their number, size, centromere position, and banding pattern. In a human karyotype, autosomes or “body chromosomes” (all of the non–sex chromosomes) are generally organized in approximate order of size from largest (chromosome 1) to smallest (chromosome 22).
Why is Acetocarmine used as the staining agent?
Stains are used in microscopic studies to enhance the contrast of specific biological components in a sample. Acetocarmine is such a stain used to stain nucleic acid inside cells. As acetocarmine specifically-stain chromosomes apart from the cytoplasm, it can be used to visualize chromosomes in mitotic studies.