Quick Answer: Which stain is used to stain nucleus and chromosomes?


What kind of stain is used for nucleus staining?

The bulk of the content inside the nucleus is nucleic acid, so nucleic acid stains are the obvious choice for nuclear staining. There are two main types of nucleic acid stains: those that will pass through the cell membrane (i.e., cell permeant) and those that will not (i.e., cell impermeant).

Which of the following stain is used to stain nucleus and chromosomes?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.

Which stain is used to colour chromosomes?

Acetocarmine and Giemsa stain can be used to stain the chromosomes.

What are the stains used to stain chromosomes and chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

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What can be used a nuclear stain?

Some dyes for nuclear staining can stain the nucleus of living cells while others require cells to be fixed and stained. When performing immunofluorescence staining of fixed cells, DAPI can be used as a nuclear stain. Propidium iodide (PI) is commonly used for measurement using a flow cytometer.

Which stain will you use to identify the location of cell nuclei?

A simple-to-use fluorescent stain, 4′,6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology.

Which of the following stain is used for staining?

Simple Stain

Basic stains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged.

What is Acetocarmine stain?

Acetocarmine is a non-specific nuclear stain which simply binds the chromosomes and gives colour to them. However, specific nuclear stain (e.g. feulgen) reacts with chromosomes to give them the colour. Acetocarmine is a dye obtained from insects.

Which stain is usually used to colour chromosomes Class 11?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

Which stain is not used for staining chromosomes?

Carmine is the basic dye used to stain nucleic acid and chromosomes and gives them pink colour. Safranin is used as counterstain in gram staining and endospore staining.