How is an electropherogram created?
An electropherogram is a plot of results from an analysis done by electrophoresis automatic sequencing. An electropherogram shows a sequence of data that is produced by an automated DNA sequencing machine. Electropherograms may be used for deriving results from: genealogical DNA testing.
What is a chromatogram or electropherogram )?
As nouns the difference between chromatogram and electropherogram. is that chromatogram is (analytical chemistry) the visual output from a chromatograph usually a graphical display or histogram while electropherogram is (genetics) a plot of sequence data obtained via electrophoresis.
What observations indicate that a peak in an electropherogram is a spike as opposed to DNA?
|What observations indicate that the peak in an electropherogram is a spike as opposed to an allele?||The spike goes through the 4 channels.|
|What steps are taken to confirm the presence of an off-ladder allele?||Re-run or re-amplify product Re-amplify sample Amplify sample w/ single-locus primers|
What causes split peaks in an electropherogram?
Split peaks are due to the main peak being split into two peaks caused by the Taq polymerase activity that causes the addition of a single “A” to the terminus of the amplified product (“N+1” band).
How many peaks for a given STR allele will a heterozygous individual have?
If there are two distinct peaks, the individual is heterozygous at that locus.
What do color and height of an electropherogram peaks represent?
A typical electropherogram from a single PCR product is shown below. Each peak represents a single nucleotide in the DNA sequence, and each nucleotide has a different colour; A is green, T is red, C is blue and G is black.