How do you study mitosis in onion root tips?

Why is the tip of an onion root used to study mitosis?

Why is onion root tip used to demonstrate mitosis in this experiment? It is because of the meristematic cells that are situated in the tip of the roots that render the most desirable and suitable raw material to study the different stages of mitosis. Onion is a monocot plant. … Hence, their root tips are used.

What can be used to study mitosis?

Complete answer: Root tips are the best to study mitosis. It is characteristic of meristematic cells present at the tip of the roots that provide the most suitable and sufficient raw material to study the different mitosis stages. Onion is a monocot plant and is the most commonly used root tip to study mitosis.

What method can be used to investigate mitosis in onion garlic root tip?

Use a light microscope to observe, identify and draw root tip meristem cells in different stages of the cell cycle. measure the sizes of some of these cells. calculate the mitotic index for the garlic root tip tissue that they observe.

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Why are root tips excellent observation points for mitosis?

The root tip of a plant contains actively dividing cells, making root tips excellent observation points for mitosis. Each plant cell is surrounded by a cell wall, making the identification of cells easy. Also, the cells near the root tip are highly organized into concentric layers, making them easy to see and count.

How do you make a temporary mount of onion root tip to study mitosis?


  1. Place the onion on a tile.
  2. Using the blade, remove the dry roots.
  3. Regrow the root tips by placing the bulbs in a water-filled beaker.
  4. After 3 to 6 days, new roots may emerge.
  5. Slide 2 to 3 cm off freshly grown roots and place them on a watch glass.

What other parts of an onion plant might be used to study mitosis?

Two specimens are commonly used by biologists to study mitosis: the blastula of a whitefish and the root tip of an onion.

Why does mitosis occur in root tips?

The gravity sensing signals in the root cap are unable to send proper orientation signals which inhibit growth in the cells that are distant from the root tip and root cap junction when kept in zero gravity. This leads to mitosis in greater number of cells and plants also produce secondary roots at a high rate.

How do you observe mitosis in root tips?

To see mitosis in action you need to look at living cells. Garlic bulbs grow roots that have actively dividing cells in their tips, in a region called the meristem. Each cell has only eight chromosomes, so it is relatively easy to see the chromosomes once they have condensed.

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Which fixative will you use to preserve the onion root tips for over a week and why?

Root tips are grown and preserved in acetic ethanol fixative. Fixed root tips can be stored for at least two weeks prior to staining. Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide.

What is fixation of onion root tip?

One of the most commonly used fixatives for root tips is Carnoy or Farmer fluid, which consists either of three parts 100% ethyl alcohol to one part glacial acetic acid or six parts 100% ethyl alcohol to three parts chloroform to one part glacial acetic acid.